Dimensions Fellow in Research

Pufall Lab, University of Iowa
Iowa City, Iowa

Aims of the Fellowship

The hope is to choose a gene that is regulated by glucocorticoids by direct binding and knockout potential glucocorticoid receptor (GR) binding cites with a CRISPR system to determine which ones regulate the gene. This includes assaying gene expression with qPCR, cloning into vectors and transfecting into cell the qPCR system, and performing a number of cell viability tests to determine if alterations in gene regulation cause treatment resistant leukemia strands to become vulnerable.

Faculty Sponsor

Brian Nowak-Thompson, Biology

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